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Mechanism of Action and NRF2 Target Engagement

Mechanism of Action and NRF2 Target Engagement

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Demonstrate the robust, specific activation of NRF2 by candidate compounds. ARE-luciferase assays (EC50/IC50). KEAP1/NRF2 protein-protein interaction assays (FP/AlphaScreen). NRF2-dependent gene and protein expression profiling. Promoter occupancy confirmation (TransAM, ChIP-qPCR/ChIP-seq)

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